Microbiology laboratory at Drug Discovery & Development Division under the Patanjali Research Foundation Trust (PRFT) is Biosafety Class–II category lab which is well equipped with Air shower, Biology Safety Cabinets (Type II, Class B2), bacteriological and BOD incubators and shakers, wall mounted and vertical automated autoclave, Flexi pumps, analytical weighing balance, pH meter, high speed centrifuges, water purification system, electric loop sterilizer equipments. Apart from these, the lab is also well resourced with some advanced tools such as colony counter Scan 1200, Microscope, PCR, UV-VIS Spectrophotometer, Anaerobic chamber, etc. Microbiology lab at PRFT is backed up by a highly skilled, qualified & experienced team of scientists. Microbiology division provides analytical support to the R&D team in line by taking care of testing of all products like Ayurvedic medicines, nutraceuticals & cosmeceutical formulations, plant extracts or any other newer formulations for their microbial profiling. Microbial profiling of test article includes testing of microbial contaminants viz; Total Aerobic Bacteria Count (TABC), Total Yeast & Mould Count (TYMC), and to test the presence of any specific pathogens viz; E. coli, Salmonella spp., S. aureus, and Pseudomonas aeruginosa. In addition to microbial profiling at microbiology lab characterization of bacterial isolates or specific pathogens by using morphological and biochemical tests which in due course followed by molecular techniques such as gene/genus-specific primer amplification.
The microbiology team also involved in multifarious activities pertaining to research and innovation in the field of Ayurvedic / Herbal Medicines, Nutraceuticals by using modern science and tools, without adulterate the principles of Ayurveda. These activities majorly include the in-vitro antimicrobial activity of Ayurvedic medicines/ formulations and or plant extracts against broad-spectrum of bacteria and fungi strains that are responsible for serious illnesses and disorders such as Skin infections, lung infections, gastric infections wound infections and Urinary tract infection, etc. Antimicrobial potency of the test article determined by measuring anti-microbial sensitivity, %Inhibition, minimum inhibitory concentration (MIC), minimum bactericidal concentrations (MBC) and minimum fungicidal concentrations (MFC). These in-vitro assays are followed to characterizing the mechanism of action of test articles by using different macromolecule approaches. Mechanism of action of bioactive substances against bacterial strain evaluated by measuring their effect on the bacterial cell wall, cell membrane, protein synthesis inhibition, nucleic acid synthesis inhibition, and bacterial metabolites inhibitions. The antifungal potency of any bioactive substance correlates by its cellulose and chitin inhibitory properties. Cellulose and chitin are the major components of the fungal cell wall.
Additionally, our in-vitro work on bio-control agents and on plant growth factors expand knowledge on plant system and give a deeper insight into the overall plant growth development that has future implications in the field of agriculture microbiology.